Backbone

The backbone takes up the majority of your plasmid. It carries all the genetic information for your GMO that isn’t directly related to the gene you’re expressing. This includes resistance to antibiotics and the replication origin which controls the number of your plasmid present in any one cell at a time.

The backbone you’ll be using is called pSB1AC3 and is the area shown in red in the map plasmid below. pMB1 is the replication origin and has a relatively high copy number of 100-300 per cell.

backbone
pSB1AC3 displays both ampicillin and chloramphenicol resistance, shown above as AmpR and CamR. This is important during the growth of the bacteria as both ampicillin and chloramphenicol are added to the agar to ensure that only cells which have taken up your constructed plasmid can thrive. The table below give additional information on these drugs.
resistance table
You can find more information on ampicillin here and more information on chloramphenciol here.