Ribosome Binding Site

The ribosome binding site (RBS) is the genetic sequence which initiates translation in mRNA. The greater the strength of the RBS the greater the average number of ribosomes that are likely to bind to the mRNA and translate it into the protein. This means the stronger the RBS the greater the number of copies of your protein will be produced.

The RBS you’ll be using is called BBa_B0030 and is considered a relatively strong RBS. Below is a graph comparing different ribosome binding sites from the same family to BBa_B0034 which has been defined as having a strength of 1.